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 Wysłany: Wto 22:54, 18 Sty 2011 Temat postu: mbt uk Rice gametophyte, sporophyte cytoplasmic ma |
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Rice gametophyte, sporophyte cytoplasmic male sterile line analysis of mitochondrial translation products in vitro
- LlI84SoteloJMDavidTHAbsenceofheatsh0ckproteinsynthesisinisolatedmitochondfiaandplas ~ dsfrommaizeJBiolChem ~ ,[link widoczny dla zalogowanych],1987,[link widoczny dla zalogowanych],262:12288 - I22925LaemmliUCleavageofstructuralproteinsduringtheassemblyoftheheadofbacteriophageT4Nature, 1970.227:680 ~ 6856 J. Sambrook Fritsch R Maniatis T Molecular Cloning (2nd edition). Beijing: Science Press. 1992r9675 ● capsule of Cai, Chen et al: Rice gametophyte sporophyte cytoplasmic male sterile line Sui analysis of mitochondrial translation products in vitro 523VariationofMitochondrial/nvitroTranslationProductsofGametophyteandSporophyteMaleSterileLinesofRiceCAICher ~ LengYANGZhengZHUYing-Guo (GeneticsInstitutePVufianUniversityIf, khan430072】 AbstraetRicemitochondriawereexwactedandpurifiedundersterileconditionsfromyellowseedlingsofgametoph) teandsporophytemalesterilelineycentrifugafioninadiscontinuoussucrosegradient. Mitochondrialproteinssynthesized / nvitrowerelabelledby (S) mefffiotaine. ItshowedthatthereweFetwopolypep ~ idas (52.OkD, 654kD] synthesizedinYuetaiB (maintainerline) andTaiyou2 【Fhybrid), buttheyweresuppressedinYuetaiA (sterileline). MitochondriaofYuetaiAsynthesizedacharacteristicpoJypepfdeof40.7kDwhichwassuppressedinYuetaiBandTaiyou2. PolypeptidesdifferentiallysynthesizedinWAtype 【A, B, F. ) Werenotfound. MaxieB (maintainerline) hadalsoacharacteristicpolypeptideof427kD. Apolypepfideof678kDwassynthesizedonlyinMaxieB (maintainerline) andMaxie63 (Fhybrid) andnotdetectedinMaxieA (sterileline) TheseobservationswerediscussedwithrefeFencetothepossiblemechanismsofCMSinrice. KeywordsRice, Cytoplasmicmalesterility, Mitochondria,[link widoczny dla zalogowanych], vitrotranslationnmductsReceivedJanua ~ 4, I998,[link widoczny dla zalogowanych], revisionreceivedMax, 10.1998ThisworkwassupportedbyNationalNaturalScienceFoundationofChina
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