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Determination of whole blood clotting time using tissue factor activity

3%, while plasma prothrombin fragment 1 +2 (F1 +2) l0 times higher than the basic level. Another group of clinical test data show that LPS stimulation of whole blood by an average of 4h, TiFaCT reduced 37.9%, plasma recalcification time was shortened by an average 27.2%. Normal in this group after LPS stimulation of whole blood clot time of 4h shortened by an average 23.9%, DIC group of 23 patients with an average reduction of 42.7%. PLS TFPI inhibition test showed a blood clot after stimulation shortened the time to be part of the inhibition of the whole, suggesting the specificity of the test. · 29.3 .2 TF-PCA factors measured in this experimental study the number of blood cells in whole blood, LPS concentration to stimulate the time TF. Detection of PCA and test repeatability,[link widoczny dla zalogowanych], and provides an ideal experimental data. This information also provides the use of PRP, PPP detect differences in TF-PCA, and that the platelet TF. Significant enhancement of PCA. Currently many reports indicate that platelets and was not found TFmRNA,[link widoczny dla zalogowanych], speculated that platelets from other cells carried by TF and thus affect the whole blood TF-PCA. The experiments show with LPS stimulated mononuclear cells in the procoagulant response to PRP than in PPP strong. Lack of plasma TF-PCA factor test results suggest a lack of intrinsic coagulation pathway had no effect on the test, exogenous pathway of coagulation factor FVII, FX had a significant effect on the experiment, because the formation of TF and FVll rrF-FVIIa coagulant that has the effect The FX is a complex substrate, so FVII, FX deficient plasma could not be detected TF. PCA. 3.3 The number of clinical research results show that the blood of patients with myeloid surface expression of tissue factor in vivo coagulation is activated, an important prothrombotic state pathogenesis. The study confirmed that TF on cells in pathological conditions,[link widoczny dla zalogowanych], LPS, cytokines can induce expression of monocyte TF,[link widoczny dla zalogowanych], in particular, LPS can induce expression of monocyte TF has been widely confirmed, and a significant procoagulant activity J . Present in circulating tumor cells with high expression of TF,[link widoczny dla zalogowanych], and produce sustained TF-PCA. Some scholars J measured by TiFaCT method in patients with unstable angina whole blood TF-PCA was significantly higher than normal blood. I check the information and suspected DIC DIC group TiFaCT shorter than normal group, indicating that the two cases of leukemia cells in whole blood increased expression of TF, TF. PCA enhanced. Thus TiFaCT check clinical symptoms before the diagnosis of DIC or DIC (pre-DIC) has important reference value.