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Effect of mifepristone on cultured human decidual cells

Progesterone and anti-glucocorticoid steroids, mainly used in termination of pregnancy, contraception and anti-adrenal cortex hyperfunction and so on. Now that RUl86 of progesterone receptor with strong affinity. Progesterone receptor formation RUB6 a complex level of specificity in the receptor blocking effect of progesterone. Cause decidual degeneration. Our animal experiment showed that the RU described the role of strong anti-pregnancy, especially anti-trimester of pregnancy to save more significant results. And prompts the decidua of pregnancy may be the role of anti RUl86 main target organs and cells _JJ. To further confirm this view. The work method of in vitro cell culture Ru. B6 in human decidual cells. To further elucidate mechanisms of drug action basis. Materials and Methods specimens obtained under sterile conditions 7 to 9 Zhou Jiankang postmenopausal women pregnancy abortion decidua (not the recent use of contraceptives with a ring of non-persons). Decidual cells in culture with reference to conventional monolayer cell culture,[link widoczny dla zalogowanych], the growth of cultured 72h select a good replacement of culture medium when cells were randomly divided into control group and experimental group. Experimental group, the concentration of RU486 were 40,80,120 t, g/m1. At 72h after dosing termination of culture, 10% formalin-fixed, hematoxylin eosin staining Seiichi. Proud of cell morphology. After the results of decidual adherent cells were seeded 6h, and continue to grow in sheets. 6d different stages of postnatal development of decidual cells closely arranged, large round to polygonal cells in or near the main. Also seen a number of stellate cells and spindle cells. Cytoplasm full, oval-shaped nucleus. Jen has multiple edges, and some cells can be seen double-edge (Figure 1). RU4B640fg / ,[link widoczny dla zalogowanych], edge contraction week, there were many cytoplasmic vacuoles. individual cell morphology were good (Figure 3). Ru120fg / Group: sharp decline in the number of decidual cells, the cell distribution of scattered and small, the cell body as slender spindle-like degeneration was ; the same time after the dissolution of residual visible cell nuclei and cell debris (Figure 4). with rings in vitro morphological changes in cultured human decidual cells in human decidual cells in the control group ①. cultured 6d. cell growth rate well HE ~ I80; 0 from decidual cells cultured plus I (40, hd) cultured 72h. and resistance to the car was similar to that HExl ∞; @ decidual cells in vitro plus I * (8o} raise 721:. cell degeneration was like HE × l00; 0 Pei Mang decidual cells in vitro increases RLk (12D) cultured 72h. degeneration of cells were scattered like l-~ x5 discuss the results of this work indicate that Ru486 in vitro on cultured human decidual cells significantly inhibited the growth. Performance New cell to reduce the number of cell degeneration and necrosis. and is enhanced with increasing dose, consistent with the results of animal experiments .... Looking RUB6 drugs in vivo and in vitro Jiuying observed results. We think: RUlB6 major role in anti-pregnancy effect site may be reported to the decidua, decidua during pregnancy and progesterone receptor most abundant [3]. Ru lizard as a progesterone receptor antagonist,[link widoczny dla zalogowanych], and the decidua through the progesterone receptor binding. in the receptor blocking progesterone levels, so that the degeneration of decidual necrosis, impede or affect the embryo implantation, termination of pregnancy. In addition,[link widoczny dla zalogowanych], Ru486 has a direct impact on the function of placental hormone secretion. chorionic gonadotropin hormone secretion hydrazone reduced luteal degeneration occurs and thus further aggravate the degeneration of decidual necrosis. Although RU486 can be one of the hypothalamic-pituitary axis of hydrazone luteal function, but in mid-pregnancy in rats within a short time (24h) have strong anti-fertility effect that decidual and ship disk drugs on the direct effect is an important factor. the mechanism remains to be further studied. Key words mifepristone; decidual cells; monolayer cell culture; vitro
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