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Chlamydia monoclonal antibody in large-scale cattle and sheep farm-related antibody detection

Chlamydia Mchb-ELISA and IHA test were compared in 50 cattle, sheep serum using two test methods for simultaneous detection of bovine serum ELISA, positive rate of 32% (16/50), IHA was 24% (12 / 50), the former is 8 percentage points higher than the latter. Coincidence rate between 88% (44/50); 50 sheep serum ELISA, positive rate of 26% (13/50), IHA was 22% (11/50) the former is 4 percentage points higher than the latter, coincidence rate between 88% (44/50). 2.2 The established ELISA method using bovine serum Chlamydia antibody test results in Table 1: Table l bovine serum showed that in 1361, the average positive rate of chlamydia 9.18% (125/1361) , the highest positive rate of the suburbs of Yinchuan,[link widoczny dla zalogowanych], was 14.12% (100/708), Guyuan lowest 0 (0 / 29). The positive detection rate was 71.43% market (10/14). 2.3 The established ELISA method using goat serum chlamydia antibody on the test results shown in Table 2: Table 2 showed that an average of 698 were positive for chlamydia in serum was 22.06% (154/698), Yinchuan I outskirts of the highest positive rate, 22.98% (2 / 396), Guyuan lowest, 15.63% (5 / 32), sheep farm was 100% (10/10). Of all ages flock chlamydia test results shown in Table 3.3 Summary 3.1 This test and discussion of 14 cattle farms in Ningxia and part of 1361 the park were cultured bovine serum and l0 a sheep farm of 698 sheep sera were Chlamydia psittaci antibody detection, the results showed: The average positive rate of cattle 9.18%, 22.06% for the sheep. Sheep were significantly higher than that of cattle, Sichuan Area 1 (Yinchuan suburbs, the South Bank area, the northern area), the positive rate in the Guyuan region in the detection of bovine serum in the suburbs of Yinchuan positive rate was significantly higher than I the other three regions, which may be animal mobile, a large number of cattle on the introduction of species; in sheep serum testing, the positive rate of sheep increased with age and increased after the age of this phenomenon in 1.5 the flock is more obvious, indicating the increase with age by increasing the odds of chlamydial infection. In recent years, Ningxia region in cattle,[link widoczny dla zalogowanych], sheep breeding barriers exist in the phenomenon of more, some cattle farm cow pregnancy miscarriage and stillbirth rate of 40% or more, and to varying degrees,[link widoczny dla zalogowanych], calf, lamb pneumonia, enteritis, arthritis and so on,[link widoczny dla zalogowanych], but the exact cause is unknown. The serological survey, cattle, sheep and cattle chlamydia in the Ningxia region, sheep infected with more common, chlamydia infection is likely to lead to one of the main obstacles to cattle and sheep breeding, the region will also population constitutes a potential threat to public health. In this survey, based on serology, from bovine fetal stomach contents in the initial completion of the separation of bovine pathogen Chlamydia work. 3.2 Development of specific anti-chlamydia McAb for the diagnosis, using ELISA, cattle, sheep serum chlamydia, the results show specific McAb against chlamydia developed a high degree of specificity and high The sensitivity, is feasible. By ELISA method and the traditional method of trial comparing HIA, ELISA was used to detect the positive rate was significantly higher than the HIA test, the two diagnostic methods was 88%. In addition to application of McAb-ELISA method than the IHA test method has higher specificity and sensitivity of the outer, ELISA method in practice also has to result from the determination of sample can be automated to reduce operation and determine the technical error, and Check the sample capacity and other more obvious advantages and convenience to help early diagnosis of chlamydia disease and epidemiological investigation.
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