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Social ,1993:16-56.2 Yin Zhen, Liu Jinghua. Animal virology [M]. Beijing: Science Press ,[link widoczny dla zalogowanych],1997:110-136 .3 KimJJ,[link widoczny dla zalogowanych], SimbiriKA, SinJId. Cytokinernolectdaradjuvantsmodulaleimmunerespons ~ inducdbyDNAi / accineconstructsHIV a 1andSIV ĄžJJ. JInterferonCytokineRes, 1999; 19 (1) :77-84 .4 H ~ akeT. VeronicaG. NeumannKd. fiveinduorionofHIV. spe-cificCTLbymulti-epitopeusinggeneguninacombinedvaccinationre-gime ĄžJJ. Vaccine, 1999; 17 (1999) :589-596 .5 G1ushako'caS, CrlvdJC, Suryw ~ yanaKddz. NefceshumanimmunodeficiencyvirubreDĄż Ąži0nand1-esl3o1.1sil/cnes8tointerleukin a 2inhumanlymphoidtissueexvivo JJ. JVirol, 1999; 73 (5) :3968-3974 .6 SasakiS,[link widoczny dla zalogowanych], TsujiY,[link widoczny dla zalogowanych], FukushimaJetdf. 1hesearchforap clear ĄŢ tDNA ~ Ine8nstAIDS: theen} umeementofimmunogenicitybychemicalandgeneticadjuvants ĄžJJ. AnticancerRes, 1998; 18 (50) :3907-3915. [Received 20o3.08.13] (music editor Li) according to the length difference and the ratio of the target sequence length increases,[link widoczny dla zalogowanych], the amplification efficiency of the influence is more obvious. Actin on the quantitative results through the correction, the total RNA between samples to eliminate the amount of RT efficiency differences and variability, so that cD-NA better reflect the differences in the level of mRNA expression changes. In summary, we construct the multi-specific internal standard template of a competitive PCR in the quantitative application, and trying to study for the next 6 gene detection of immune effector molecules. 4
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