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Wysłany: Czw 0:05, 10 Mar 2011 Temat postu: Aeromonas disinfectant resistant gene of _746 |
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Aeromonas disinfectant resistant genes
The detection rate of 19 due to multiple drug resistance of Aeromonas was detected in a sull Yang sad qacEA1 7 Jurassic,[link widoczny dla zalogowanych], the positive rate was 36.8%, including 3 strains of Aeromonas hydrophila, Aeromonas two guinea pigs bacteria, moderate and Weilong Aeromonas Aeromonas strains of the l. 2.4qacEA1 gene PCR product of a sull electrophoresis (see Figure 1) Figure 1qacEA1 a sull gene PCR product electrophoresis Fig1ElectrophoresisofPCRproductsofqacEA1 a sullgene Note: M: molecular weight marker, from top to bottom,[link widoczny dla zalogowanych], respectively 50,100,150,200,250300, 400500600700,8009001000 bpP: positive control, N: negative control, S: sample positive for 3 to discuss the concept and disinfected with sterile universal consciousness, more and more extensive application of disinfectants. More common applications is inefficient disinfectant,[link widoczny dla zalogowanych], for the cationic surfactant, has the dual role of disinfection and decontamination. Disinfectants to kill bacteria through the body, fungi and lipophilic viruses, so as to achieve disinfection requirements, such as benzalkonium bromide disinfectants, including quaternary ammonium salt such as benzalkonium bromide, clean off Seoul, Domiphen; biguanide disinfectant such as chlorhexidine Thailand and so on. Widely used in clinical wound disinfection, eye, bladder, vaginal washing disinfection, surgical preoperative skin, mucous membranes and doctors hand disinfection; in everyday life, such as paper, disinfectant wipes, hand sanitizer, liquid, also the application of Jieyin more to more. Widely used disinfectant bacterial resistance to disinfectants, disinfection leaving invalid. Disinfectant resistance gene family expression in a variety of compounds qac efflux pump genes and expression in bacteria can be quaternary ammonium compounds, biguanides such as excluding compounds outside the cell. qac gene family have been found to have A, B,[link widoczny dla zalogowanych], C, D, E, EA1, F, G, H,[link widoczny dla zalogowanych], Jl0 species. Foreign scholars have been found in bacteria such as Staphylococcus qacA, qacB gene, and multidrug resistance of MRSA of qacA, qacB gene was higher than that MSSA0 '. Density, etc. in support of domestic yellow Enterobacter cloacae, Pseudomonas aeruginosa, Acinetobacter spp, Stenotrophomonas few oxygen detected in Aeromonas qacEA1 genes. The first time in the multiple drug resistance in Aeromonas found qacEA1 a sull gene, qacEA1 disinfectant efflux pump gene expression, and sull genes resistant to sulfonamides, the two often overlap occurs, qacEA1 a sull gene that carries both positive disinfection agent resistance and sulfonamide resistance genes. Aeromonas sull production qacEA1 a positive rate of 36.8%, distributed in various Aeromonas species, that is not sterilized is safe, long-term application, bacteria resistant to disinfectants may also be a result of disinfection is not valid, it is recommended the best choice for several different types of cross-use of disinfectants can delay drug resistance. At the same time reminds us that multi-drug resistant bacteria resistant bacteria may be a predictor of disinfectants, should arouse the attention of workers in infection control and the need to further study.
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