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njlpkhgea
Wysłany: Sob 12:06, 19 Mar 2011
Temat postu: Smoked on the sulfur content of yam polysaccharide
Smoked on the sulfur content of yam polysaccharide
5rain, out, cold set at room temperature, absorbance was measured at 490nm wavelength, the concentration of C as the abscissa, absorbance A for the vertical axis, the standard curve, the regression equation is: A = A 0.0337 +10.225 C, r = 0.9999. 2.5 conversion factor of 60% measured accurately weighed dried to constant weight of the yam polysaccharide 10mg,
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, home 25ml volumetric flask, add distilled water and diluted set the scale, take 5ml, set the volume to 50ml, according to Preparation of standard curve was determined under the absorption, polysaccharide obtained from the regression equation for the concentration of glucose in the test solution C, calculated as conversion factor f = w / (CXD), where w is the weight of polysaccharide (mg) ,
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, C is the concentration of glucose in diluted polysaccharide (g · L), D the dilution factor for the polysaccharide. f = 1.421, RSD: 2.6% (n = 5). 2.6 The precision of precision drawing test solution 2ml, 5 copies, under the standard curve method is obtaining the average percentage measured = 17.9%, RSD = 0.3% (= 5). 2.7 Stability test of the above mentioned raw materials for the operation of the test solution by method of determination, an absorbance measured every 30min, 3h stable within the measured values determined. 2,8-recovery experiment, accurately weighed 1g yams, home Soxhlet extractor, add ether degreasing, solvent evaporated to dryness, add 80% ethanol Soxhlet extraction to remove monosaccharides, oligosaccharides and glycosides. After the solvent evaporated to dryness dregs, adding glucose and distilled water extraction method according to the test prepared for the test solution. Determination of the remaining items the same operation method, the measured rate = 99.60%, RSD = 1.48% (= 5). 2.9 will be the provinces of determination of sulfur smoked yam sample solution before and after, respectively, precision drawing 1ml, home 5Oml flask, distilled water volume, the same method to analyze measured absorbance A measured concentration of C by the regression equation . Content and then calculated as follows: polysaccharide content = CDf / W × 100%. Where C is the concentration of glucose test solution (g · L), D is the dilution factor for the test solution, f is conversion factor, w is the weight of the test (mg). The results in Table 1. Table 1 yam Determination of polysaccharide in the sample 3 to discuss the results of this paper,
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, defatted with ether, first with 80% ethanol to remove monosaccharides, oligosaccharides and glycosides interfere with alcohol-soluble components such as to prevent it from becoming polysaccharide impurities,
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, and then water extraction of polysaccharides. Polysaccharide is a major component of yam, possess immunomodulatory,
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, hypoglycemic and anti-tumor effect, while the polysaccharides can not be directly determined. In this paper, under the action of polysaccharides in the sulfuric acid hydrolysis into single sugar molecules, and quickly dehydrated to furfural derivatives, and phenol compounds produce brown, then colorimetry. The results showed that: the phenol sulfuric acid assay is simple, for the test solution color stability, good reproducibility, recovery was 99.6%. This method is suitable for the determination of polysaccharides in yam. Smoked with the amount of sulfur in the polysaccharide content of yam can increase, but continue to increase the amount of sulfur, the polysaccharide content declines and exclusion of the system a long time system of yam polysaccharide content shorter than the exclusion of the yam polysaccharide content is low. The amount smoked by the maximum sulfur content of yam polysaccharide and polysaccharide content of other provinces are still very yam. During the experiment, found that storage time after mixing and heating time on the color of the larger, generally placed after mixing 5rain, cooled to room temperature after heating 15rain, the determination to get better results.
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