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Effective use of capillary electrophoresis separation of 12 anions

Product has the same effect, but the cost is much lower than imported products, greatly reducing the operating costs capillary electrophoresis. Meanwhile, the effects of different buffers on the separation of several common anions, migration time and peak area reproducibility, recovery and detection limit of 12 anions and other issues. 2 Experimental 2.1 Instruments and reagents DIONEXCES-1 capillary electrophoresis; UTOSCIENCEAP_01 vacuum pumping filter; SCQ-250 ultrasonic cleaner; N2000 chromatography workstation. 75pan × 55cm silica capillary into the sample buffer solution of gravity height of 10cm,[link widoczny dla zalogowanych], the sampling time of 30s, Used deuterium lamp detection, indirect UV detection at 254nln. Sodium chromate (AR), the electroosmotic flow modifier TrAB, the potassium or sodium salt anions tested, self-ION-specific buffer solution solution I: 0.012mol / L sodium chromate +0.02 moVLTrAB +0. 01moVL acid; Waters Corporation buffer Ⅱ: chromic acid / i ~ / OFMTManion-BT,[link widoczny dla zalogowanych], 1moVLHC1, 1moVLNaOH, 12 mixed standard solution of anions: Br ', cl a, I', s. , NO;, eo: ', NO;,' OOCCH2COO. , F. . OOC (CH2) 2CO0. , CH, COO. And CH3CH2CO0 '. Experiment, all reagents and samples are used 0.45p. m membrane filter, buffer solution with ultrasonic cleaning, degassing 10min. 2.4 capillary experiments with acid, alkali, water cleaning activation, and then wash buffer solution and stability testing after about 25min. Each run with a buffer solution before washing 2min, after all the cleaning and testing procedures for the process of setting done automatically by the capillary electrophoresis. N2000 automatically collect, process data, using external standard method, the migration time of the qualitative to the quantitative peak area or peak height. 3 Results and discussion 3.1 Optimization of buffer solution in the buffer solution in this experiment made the recipe more try, by continuously adjusting the concentration of each component within the formula and the pH value of buffer solution I finally determine the best choice, under the conditions of the buffer anion can have 12 minutes left, the best separation. I will be home-made buffer solution buffer solution with Waters Corporation Ⅱ for comparison, in other experimental conditions are the same separation and detection of 12 anions and found that: the separation of the two the same buffer, and 12 anions in the 3.5min time completely separated, according to the order of migration time were Br. , C1. , I. , S. , NO;, eol. , NO;,. OOCCH2CO0. , F. . OOC (CH2) 2CO0. ,[link widoczny dla zalogowanych], CH3cO (). , CH3CH2CO0. . But I made the cost of buffer solution is far lower than the import buffer Ⅱ. 3.2 The results show that the choice of separation voltage, 20kV voltage in only 11 isolated anion. Which NO; and sol. Can not be separated. Increase the voltage to 25kV, 12 complete separation of anions, have also accelerated the rate of ion migration. When voltage is 30kV. The ion mobility is faster, but the baseline noise is relatively larger. So. Voltage is determined to 25kV. 3.3I and the detection limit of selection is C1. , F. , Sol. ,[link widoczny dla zalogowanych], NO; 4 anions do reproducible experiments. DIONEXCES. 1 Detection of anions, the migration time relative standard deviation less than 0.5% relative standard deviation of peak area were less than or equal to 5.0%. 12 anion detection limits were: Br. 4.0m # L, C1. 2.0m # L, I. 8.0m # L, sol. 1.0m # L, N00.5mg / L, Po =. 4.0mg / L, NO3-0.5m # L,. OOCCH2COO. 2.0mg / L, F. 1.0m # L,. OOC (CH2) 2CO0. 4.0m # L, CH3CO0'2 .0 m # L, CH3CH2CO0. 4.0m # L. DIONEXCES · 1 test anion concentration can reach a very wide range to cl. Example, the quantitative detection range of 2.0 ~ 2000mg / L in, and fast analysis, small amount of sample. 3.4 The recovery of water samples in this study, we used a common C1. , F. , Sol A, NO; do recovery experiment. DIONEXCES. 1, the recovery rate of detection of anions in 90% to 102%, respectively. Received 2003-07-14; 2004-01-04 accepted
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