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 Wysłany: Śro 22:40, 26 Sty 2011 Temat postu: Tara _2653 tissue culture technology |
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Tara Tissue Research
228 Woody plant tissue culture and application [M]. Beijing: Higher Education Press, 1986AStuayOiltheTissueCultureofTara (CaesalpiniaspinosaKuntze) L / Zhi-guo,[link widoczny dla zalogowanych], delete Hao,[link widoczny dla zalogowanych], YANGWen-yun, XIADing-jiu (TheResearchInstituteofResourceInsects, CAF, J (Immj ismainlydistributedinthevalleyareainthemiddleoftheeastAndesandintheseashoredesertalongthePacificOcean.Theplantwassuccessfullyintroducedtothexerothermicandsemi.xerothemilevalleysofYunnanprovinee, Chinain1991.Thispaperdealswiththetechniqueoftissuecultureoftara.Thereisrichtanninintaraexplantbecauseitstissuecultureisverydifficulty.Usingtenderstem,[link widoczny dla zalogowanych], stemneedle, leaforleafstalkactasexplantcultivatecallusmasstocultivatecallusmassandtouseMS + BA1.5lI】 Ig · L A + canesugar30g'L a + gagaragar5.7g. L ~ actassproutpolarizationculturemedium.thepercentageofcallusmasstosproutisonly50% .Using1/2MS + BA0.1mg . L A + canesugar20g'L a + gagaragar5.7g. L a andMS + BA1.5nag 'L A + casein1.5gL a +0.2 nag'L a + canesugar30g · L A + gagaragar5.7g' L a actasculturemediumtocultureasepsisyoungseedling10daysandtoculturecallusHlassabout20daysrespectively.thereare79 % callusmassthatcomefromhypocotylcanbegrowudplantlets.Tocultureasepsiudplantletabout20days, Using1/2MS + NAA3.0rng · L A + L1.0mg 'L a + KT0.5mg. L A + canesugar15g'L a + gagaragar5.7g' L a actasgrowingrootculturemedium,[link widoczny dla zalogowanych], thecentofgrowingrootbudplantletis76% after15clays.Theexperimentconditionisthatil.1uminationis2000 ~ 25OOlxandthetemperatureis25 ~ 29 ℃. Keywords: Tara (Caesa / pin / aspinosaKuntze); dssueculture; inducedrooting
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