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Lentinus PPO activity in different cell age Comparison

Axis of the important pieces of catalytic Jing. Unfortunate different by the end of the main speech of the mushrooms abnormal multi-Qi Yan wire oxidase activity were measured, show a series of multi-Qi Qi lace mushrooms variation of enzyme activity. Materials and methods Section 7402 strains tested by the Shanghai Academy of Agricultural Sciences mushrooms Mushroom Institute. Mycelium Chong body medium (PDY) of 20 potato sprouts recipe, 2 glucose, 0.2 yeast extract. 250 ml flask repackaging 3O ml. Samples inoculated once every l0 days repeated every forty, the same amount of joy then Korea. 25 ℃ Hengqiang training. Hyphae by different cell age 561oI washed with distilled water after filtration, filtration, plus amount of quartz sand and L, 2TBE sample buffer. Frozen ground. 10,[link widoczny dla zalogowanych],000 rpm / 10 minutes separated the heart 'to do the supernatant yam intracellular enzyme samples. Samples were dark refrigerator at 4 ℃ save backup. Phenoloxidase activity was measured by 2B ℃ constant temperature substrate reaction (Table I). Those without a substrate for the enzyme blank control,[link widoczny dla zalogowanych], using a 754 UV spectrophotometer v Determining the risk and automatically print the light values. Lo0 eggs with the enzyme activity itself every 30 minutes, said optical density change. Bradford protein determination method. Take Im] Bradford test Jing, 4ml filling Wing Yan, absorbed 100 tax enzyme solution, in 5gSmm, the brightness than color, body. Mycelium had received sugar. Market extracellular enzyme sample solution as bovine serum albumin do mycelium after the standard curve. He declined an ill-phenol Chlorine Institute, said alcohol in the reaction system (1) * the imaginary axis with Stanford B solution with 95. F (2) Confucius set degrees are g8 ℃ force results and analysis of different cell age of the mushroom mycelium of different phenol oxidase activity (Table 2), can be seen in the early mycelial growth. Extracellular enzyme activity is low. Mycelial growth is restored, start to adapt to the environment of toxic Ii, to flourish in the late growth, enzyme activity also greatly. I-strong. Technical quality of a large number of decomposition, hyphal growth required to meet the carbon nutrition. Z different caries decline Cao Cao Ji mushroom fan phenoxy pupil he lay wing of ammonia soil activity of sweet wine poured wood ■ Grand Stanford Stanford for a long time director of chloride than the Stanford 'yao) outside chest chest chest Ran Ran fish outside the chest outside the Kui Gu Gua cents within the j the 10O067m060m5g $ m21O0.2160.0ST0.85302E200.1390.0488.9Z30.4530.8170.295O. 846D. 182 300. Gamma 60052L9O. 2O. 67mll01.0360.103 grapefruit n, 0 0,0 Chi l_ projects aiming l7n. $ 34O. 9730.1 ¨ O. Pot 30.212500. II0. O593. Cricket 30. Z. Z are m2260.6Z0mZl1 with the extracellular enzyme activity was significantly greater than the intracellular enzyme. Mushroom mycelium that carbon metabolism. Secretion of the enzyme has a strong ability to participate in extracellular lignin biodegradation, in order to obtain carbon nutrition needed for growth. However, different strains of mycelium cells age chlorinated phenols uplift forces of the enzyme is different. 20-day-old bacterial enzyme secretion lao strong points such as day-old bacteria more polyphenol oxidase strongest. 6O-day-old bacteria secrete the strongest phenoloxidase more fencing. This shows that the mushroom mycelium growth process. Regulation of all hyphal cells with the ability to secrete polyphenol oxidase, and polyphenol oxidase is a margin of ten units system,[link widoczny dla zalogowanych], the biodegradation of lignin phenol oxidase from a variety of steps to complete rumors. Third, casein acid enzyme is swaddling clothes chlorinated phenols j given four intracellular enzymes of extracellular enzymes, including a minimum difference. Show that the enzyme is not nitric acid casein to participate in the biological degradation of extracellular lignin, but also to participate in the carbon floc intracellular metabolism. That tyrosinase degradation of lignin raw cabinet late active enzyme. Research into the class to be. 600. d86ml0z ●. 6z5I,[link widoczny dla zalogowanych], 0302.0 Head z0. zl105 out 036 lead II, dating back four given by phenols of chloride intracellular enzymes including, but not the extracellular end of support dates Note bar, Shanghai Institute EPRI core dump Lei Qiang Pan Yingjie charge of the students that so thanks. ~
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