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Peach leaf poisoning test

netieglycoside), also known as cyanogenic glycosides,[link widoczny dla zalogowanych], cyanide is the aglycone and sugar moiety of the condensation of the glycosides, widely found in some plants, such as apricot, peach, plum, etc. in the kernel. Glycosides could be the role of enzymes or acid hydrolysis under the conditions of heating two parts sugar and aglycone. Cyanogenic glycosides are hydrolyzed in the same time, the aglycone is also hydrolysis to produce hydrogen cyanide, resulting in toxicity. Cyanogenic glycosides poisoning usually vomiting, nausea, abdominal pain, headache, dizziness, palpitations, rapid pulse, weakness, drowsiness, slow breathing after the first frequency rate, pale, sweating out the antelope, seizures, difficulty breathing, restlessness, heart speed up, mydriasis, unresponsive to light or disappeared, and coma, physiological reflex, hypoxia, shock or respiratory failure and death. Cyanogenic glycosides poisoning test, the current indigenous plants to use more direct test, cyanogenic glycosides are soluble in water, soluble in hot ethanol and ethyl acetate, usually water extraction, samples taken mashed aqueous extracts, and then sub- water immersion for inspection. Test methods commonly used Prussian law and sulfuric acid method. In this experiment, we found in the peach leaves and other plant components also cyanogenic glycosides, animals can cause poisoning after eating. Examine application of Prussian blue, hydrogen cyanide was detected in leaves from peach ingredients, and animal experiments, guinea pigs fed with peach leaves, poisoning efforts in guinea pigs have also been detected in the isocyanate component of oxygen. 1 Materials and methods 1.1 Reagents and reagent FeSO, a NaOH test strips: the filter paper immersed in 10% FeS solution, remove and dry before use plus a drop of 10% NaOH solution, then wet. Lead acetate cotton: the cotton wool soaked in 5% skim lead acetate solution, drying stand out. 10% HC1 solution: diluted with concentrated hydrochloric acid. 10% s solution: concentrated sulfuric acid dilution. Guinea Pig: Take the healthy adult guinea pigs, male and female do not count, weighing about 250 grams. All reagents were of analytical grade. 1.2 Experimental Methods 2 grams of crushed samples, add 5 ml distilled water, placed in flask, add 10% HC1 was adjusted to pH 2 and immediately cover the mouth of the bottle strip FeSO a NaOH, heating to l5 minutes of boiling and the micro-exhaust, heat, and remove the filter paper immersed in 10% H2S04 solution, such as filter paper was blue stain, was positive. 2 Results 2.1 Experimental results and discussion to take leaves of peach,[link widoczny dla zalogowanych], peach old leaves, unripe peach, cherry leaf of Ginkgo biloba and the test method, such as 1.2, with sodium cyanide as a positive control, while as negative according to the} 『3 experiment; results, positive control, peach leaves, cherry Ye Chengjiang positive, peach old leaves, immature peach positive, ginkgo biloba, a negative control was negative. Peach leaves was more responsive than the old leaves of peach,[link widoczny dla zalogowanych], peach leaves that the content of cyanogenic glycosides in the old leaves more than peach, cherry leaves have cyanogenic glycosides composition. 2.2pH choice of the value of the cyanogenic glycosides to be hydrolyzed in acidic conditions, heating, take the same peach leaves, adjust pH value of 2 and 5, respectively, in accordance with 1.2 method test results pH2 than pH5. Hydrolysis also takes time, so the need to micro-boiling conditions for 15 minutes. 2,3 Determination of cyanide in guinea pig tissues taken four guinea pigs, after 24 hours of fasting, feeding peach leaves, and 12 hours later, three of them died, the other survived. Guinea pigs died of abdominal bloating, heart-shaped bright red flow, a small amount of blood clot. Whichever is the stomach contents were, effort and heart, liver, spleen analysis by 1.2, the results in Table 1. Table l of cyanide poisoning in guinea pig tissues determination results can be seen from Table 1, poisoning in the guinea pig stomach contents were detected in hydrocyanic acid composition, effort, and hydrogen cyanide were also detected in heart tissue elements. Not detected in the liver and spleen hydrocyanic acid composition. Generally believed that the cyanide into the body, and rapidly absorbed and distributed in the organs, the poisoning death of stomach contents and blood, because the volatile hydrogen cyanide, if not test, may lead to detection. Are of the cryopreservation of biological samples that may extend time limits in vivo detection of hydrogen cyanide. 3 guinea pigs died of poisoning after l8 samples in a test two weeks after cryopreservation, a hydrocyanic acid component is still detected. 3 Application of a spring, just northwest of a zoo monkey how the sudden death of unknown causes at the same time, the autopsy and microbiological, toxicological and other tests, does not identify the cause of death. After we use this law, after examination, the stomach contents of the golden monkey in the death of hydrocyanic acid composition were detected, from the morphological analysis of stomach contents contain peach leaves. With breeder breeding records. Confirmed by a large number of golden monkeys poisoning after eating peach leaves.
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