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Dołączył: 21 Lut 2011
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 Wysłany: Śro 1:35, 23 Mar 2011 Temat postu: 肝细胞中人 |
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肝细胞中人巨细胞病毒抗原的免疫组化检测
echniquesinthediagnosisofeytomegalo~roscolitisAmJClinPathol，1988l89}686(1995—03一O9收稿．1995一O6O9修回)编辑苏长锥ImmunohistochemicalDetectionofAntigenofHumanCytomegalovirusinHepatocyteXiongYingLiWoiruYaoYujia’ChertJunjieTanJiansan1．Dtpartm~tPediatrics(Chengdu610041)}2．R~6inantDNALaboratoryl3l~epartmentPathology，SchoolpublicHealthAl~tractTheexpressionplasmidofhumaney—viralhepatitishistopafh010gical1y．Ofthese8cases，7tomegalovirus(HCMV)52kdglycoprotelnwasin—hadpositives~gnalsbuttoundinthenucleusandcy-ducedandamplified．andtherecombinantproteinwastomemhraneofthehepatocytes，nopositivesignalwasextracted．Therahhitantiserumtotherecombinanttouodinthehepatocytesofthecontro1．Theresultproteinwasprepared．TheantiserumreactedtotheshowedthatHCMV52kdglycoproteinwaslocatedjnantigenofHCMVADIⅢandthetlterofantiserumthenucleusandonthemembraneoftheinfectedhepa—was1l3200byusing”I-ProteinA．UsingAvidin—tocyte．TheantibodypreparedwiththeantigenofBiotinperoxidasecomplextechnique(ABCmethod)-geneengfneerfngcouldfindthevirusantigenandrep—wedetectedtheHCMV52kdglycoproteinintheresentthevirusgenestatewhichwasactiveorsilent-haPaticslidesof9cases．Amongthem．onecase(anABCmethodisnotonlysimple-sensitiveandspecific，inf日ntdiedofcongenitalheartdisease)washeldasahutalsoeffectiveinlocalization—Itisofimportancetonegativecontro1．Theother8cases(patientswithclinicaldiagnosis-differentialdiagnosisandprognosti-clinicallydiagnosedcytomegallcinclusiondisease)hadcation—KeywordsHumancytomegalovirus52kdglycoprotelnImmunohistcehemiealdetection
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