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Enhanced horseradish peroxidase catalyzed luminol chemiluminescence reaction of sodium tetraphenylborate new enhancer

And the luminous intensity of a good linear relationship. The linear regression equation is Y a 0.62g +7.825 (HRP) with a detection limit of O. 6 × 10nmol / L. On the o. 6 × 10mol / L of HRP for l1 relative standard deviation of parallel determinations was 1.54 Conclusion enhancer widely used at present have a similar structure,[link widoczny dla zalogowanych], namely with 1 to 2 substituents of aromatic ring compounds. This new type of enhancer used in the past used NaTPB enhancer has a completely different structure and characteristics of the central atom is coordinated boron and four benzene rings are composed of structurally different from the above, with more benzene to form big button from the field, the electron transfer easier. The detailed mechanism remains to be further explored. This new enhancer that could lead to a class of similar structures in the development and application of enhancer for the HRP labeled CL enzyme immunoassay provides a new measuring system, and the reinforcing agent soluble in water,[link widoczny dla zalogowanych], unlike phenol class and other organic substances,[link widoczny dla zalogowanych], which in the determination,[link widoczny dla zalogowanych], conservation and development kit has a special advantage.
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