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Caused by Staphylococcus aureus with food poisoning

The health facilities, poor environment, employees were not physical examination, poor personal hygiene, food and debris piled up together. March 20, for two days of continuous snow fall, more people buy lunch, Liu boxing as early as eight cooking, then quilt Gaiyan, 12 sold out, sold a total of 33 boxes, according to some students reflected. Feel stupid ignorant of the meal, 1-2h after a meal, the five students have varying degrees of nausea, vomiting, headache, dizziness, abdominal pain, diarrhea and other symptoms, Liu Ruiying have the students take Patchouli upright in water, but did not improve , also have been admitted to hospital soon after treatment to relieve symptoms of the three students on the same day and discharged. It is understood that each student have breakfast with their families at home, eat different foods, but at the same time, parents no one similar food poisoning symptoms, so the students can rule out the possibility of food poisoning eating at home. If not with other food, eat lunch is the review circle Man 2000No. 8 (Interest 194) feet f Rainbow O Du Hongmei (Qiqihar Shifangyizhan) measurement focus. 2 Laboratory test results 2.1 21.1 Materials and methods Laboratory examination material I stood in the evening of 20 March 1998 5:55 received preventive health care, Second Affiliated Hospital of case reports of food poisoning, admission 5 people, no death. Speed to Jianhua Liu Ruiying lunch for two processing plants on-site sampling, collecting samples: 1. Lunch; 2. Spatula; 3. Dried soy bean curd; 4. Mixed vegetables (pot package of meat, eggplant, potato silk, mushroom); 5. Box meal; 6 lunch; 7 feces; a total of seven pieces. Speed back to the laboratory for microbiological testing 2.1.2 reagents MM, GN, SF, broth, fermentation of lactose bile tubes, EMB, SS, ordinary nutrient agar plate, blood agar, Staphylococcus-specific medium plate, triple sugar iron agar slant tubes, mannitol fermentation tube, 38% of anticoagulation, 20% sulfosalicylic acid, 0.04% BTB. 21.3 to 1 to 7 samples were inoculated into MM, GN, SF, broth and alkaline peptone water in 37q [, 24h enrichment program after the SS. EMB, blood agar, ordinary nutrient agar plate, medium plate special Staphylococcus, 37qC, 24h after 3, 4,[link widoczny dla zalogowanych], 6, SS. EMB ordinary nutrient agar plates were picked smooth surface moist, medium-sized colony, inserted Clinton Overseas Double sugar iron agar slant tubes, in the 3.4.6 private plate number aureus colonies were picked yellow dye into the G, the results for the G cocci, arranged in one. Poisoning. Li, the blame will be a food from a simple satin Li 2000No7 (total 193) Grape-like, no spores, no capsule, in the blood agar colony No. 3.4.6 was white,[link widoczny dla zalogowanych], large and protruding opaque,[link widoczny dla zalogowanych], smooth surface, colonies hemolysis around the ring. 2.1.4 biochemical identification: serological identification and coagulase tests: 2.1.4.1 lessons 1mI38% of anticoagulation with 4mI rabbit blood mixing, then centrifuged supernatant. 2.1,4.2 drawn rabbit plasma supernatant 0.5mI, pick a single colony and plasma grinding yellow uniform. Apparent particle agglutination for the 3.4,6 2.1.4.3 draw three drops of sample 0.04% BTB drops in the number 3.4.6 Staphylococcus dedicated yellow colonies on plates, colonies appeared around the yellow , mannitol (+). 2.1.4.4 draw drops of 20% sulfosalicylic acid in Staphylococcus aureus-specific yellow colonies on the plate No. 3.4.6, there hemolytic colonies surrounded by a transparent, liquid gelatin. According to epidemiological investigation, clinical performance and test results, you can determine this from the poisoning, Staphylococcus aureus in food poisoning. 3 the results are not achieved 3.1 Liu Ruiying lunch plant health permits, processing and sale of lunch without permission, according to use,[link widoczny dla zalogowanych], and destruction of the remaining suspect food. 3.2 The production process does not meet food hygiene requirements. Five students were caused by bacterial food poisoning (Staphylococcus aureus poisoning),[link widoczny dla zalogowanych], according to 4 Strategies to strengthen school health and views 4.1 Comprehensive law enforcement efforts. Further improve the legal awareness of school leaders to enhance the students knowledge of food hygiene law advocacy, monitoring the formation of a good atmosphere. 4.2 to further strengthen the Improve training for food business operators to further enhance their professional ethical standards. 4.3 strengthen law enforcement, strengthen supervision and monitoring of the frequency of violations must be strictly dealt with expeditiously and ensure people's health.
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